cell culture flask中文是什么意思

中文翻译手机版

In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
方法：胰岛的分离参照王玲等的方法，每次实验取新生3 - 5天sd大鼠，无菌条件下剖腹取出胰腺，剪切为0 . 5mm ~ 3的组织块， v型胶原酶消化30min后，离心洗涤，悬浮于完全培养基，接种入50ml培养瓶，于5 co _ 2 、 95空气条件下培养20h ，转板纯化，接种于96孔培养板培养24h ，按实验要求进行实验。

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